Roswell Park Memorial Institute 1640 And DMEM/f-12 — A Perfect Guide
The Roswell Park Memorial Institute is an inspiration behind the name-after-medium that Moore et al. devised, RPMI 1640. Ideal for cultivating non-adherent cell types such as lymphocytes and other blood cell lines. A balanced salt solution (BSS) based on RPMI 1630 series of media is called RPMI 1640.
The pH is kept neutral with the help of a bicarbonate buffering system, and the quantity of vitamins and amino acids from RPMI 1630 medium is changed. Some cell lines require proteins, lipids, or growth factors, which are lacking in RPMI 1640 medium. As a result, FBS may need to be added to the medium for cell lines to develop and proliferate.
In this blog, we will explore RPMI 1640 and DMEM/f-12 in detail.
Let’s Begin!
What is RPMI 1640 Media used for?
● RPMI 1640 has long been used to cultivate human lymphocytes and expand human lymphoid cells without serum.
● When appropriately supplemented, RPMI 1640 proved to be very applicable for cultures of various mammalian cell types, including cancer cells like HeLa and Jurkat cell lines as well as blood cells like monocytes and fresh human lymphocytes.
● Because of its adaptability, it can be used as a good basal medium for laboratory cell culture as well as for research purposes, including drug development, vaccine development, and cell-based therapeutics.
● A type of medium used in tissue and cell culture is called Roswell Park Memorial Institute (RPMI) medium, or RPMI 1640. It is used to grow human lymphocytes as well as a range of animal cell lines.
● A balanced salt solution (BSS) based on the RPMI 1630 series of media is called RPMI 1640. The pH is kept neutral with the help of a bicarbonate buffering system, and the quantity of vitamins and amino acids in RPMI 1630 medium is changed.
RPMI 1640 vs. DMEM
● RPMI and DMEM are two popular mammalian culture mediums, but they differ significantly in a few important ways. Glutathione, a reducing agent, with high amounts of vitamins, and choline is exclusively found in RPMI 1640 Medium. In addition, it has PABA, biotin, and vitamin B12, all of which are absent from dmem/f-12.
● Another distinction between the two media is that they are formulated for different types of cell culture. Whereas mammalian cells are cultured in suspension culture using RPMI 1640, adherent cells grown on surfaces like plastic or glass are cultured in DMEM.
● Whereas RPMI 1640 is used to culture mammalian cells in suspension culture, DMEM is utilized to culture adherent cells grown on surfaces like plastic or glass.
● RPMI is a medium widely used to culture mammalian cells in suspension culture, whereas DMEM is a modified type of basal medium with increased amino acid and vitamin concentrations.
What is the composition of RPMI and DMEM?
● The concentration of glucose in DMEM (low glucose) is lower than that of RPMI 1640 (2 g/L) at 1 g/L. Furthermore, compared to RPMI 1640, which has 0.8 mM of calcium and 5 mM of phosphate, DMEM has a higher concentration of calcium (1.8 mM) and a lower concentration of phosphate (1 mM).
● RPMI 1640 includes two grams of glucose per liter. A 5 mg dose of phenol red as a pH indicator 6 grams of sodium chloride, 2 grams of sodium bicarbonate, 1.512 grams of disodium phosphate, 400 mg of potassium chloride, 100 mg of magnesium sulfate, and 100 mg of calcium nitrate are among the salts.
● The concentration of vitamins and amino acids in Dulbecco’s Modified Eagle Medium is four times higher than that of the original Eagle’s Minimal Essential Medium. Sodium pyruvate and glucose were present at low concentrations (1 g/L) in the initial DMEM formulation.
● The growth of several cell types can be supported by DMEM and RPMI 1640, which were utilized in this investigation. There are certain variations in their composition, though, and these might have an impact on the survival, differentiation, and proliferation of cells.
How to choose the right RPMI 1640
● While choosing the appropriate Gibco RPMI composition for your cell culture research, you need to take a few things into account: format, supplementation, and additions.
● With the help of our interactive media formulation tool, you can quickly select the RPMI 1640 that you require by comparing Roswell park memorial institute 1640 formulations side by side.
- Supplementing with L-glutamine and GlutaMAX
- Phenol red light
- Formats: liquid versus powder
- Supplementing with serum
- Adding sodium pyruvate
- Additives made of glucose
- HEPES supplement
